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Technical article · 2025

Probiotic wafers in aquaculture: Innovative application in shrimp farming

Ermel Viteri Santana, Marcos Santos, Jan Koesling, Andrew Shinn

Probiotic wafers delivering concentrated Bacillus strains significantly enhance Penaeus vannamei broodstock performance, nauplii output, grow-out yields, and pond biosecurity.

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Abstract

Traditional powdered and top-coated probiotics face operational constraints such as dosage inconsistency and leaching. This article evaluates the development and commercial efficacy of slow-sinking, water-stable probiotic wafers (Sanolife PRO-TAB) containing Bacillus subtilis, B. pumilus, and B. licheniformis (>2 × 10^9 CFU/g). Commercial trials in Ecuador with Penaeus vannamei broodstock receiving 0.4 g of probiotic wafers daily demonstrated marked reproductive gains: survival rates of non-ablated females significantly improved, daily copulation events nearly doubled from 5% to 9%, egg production per female rose by 6%, and total nauplii production increased by 37%. Treated offspring exhibited lower pathogenic bacterial and Vibrio loads throughout hatchery cycles. In Thailand grow-out ponds (22 ppt salinity), wafer-treated ponds reached harvest in 98 days versus 102 days for controls, achieving 14% higher yields. Nursery trials showed economic profit improvements ranging from 12% to 54% above standard practices. The wafer format enables targeted pulse delivery, stimulates ingestion even in challenged animals, supports dual gut-environment bioremediation, and simplifies daily farm protocols.

Keywords

probiotic wafers, Penaeus vannamei, Bacillus subtilis, broodstock performance, nauplii production, Vibrio control, shrimp aquaculture

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