Scientific paper · 2025
Siriwan Khidprasert, Chanadda Kasamechotchung, Rungkarn Suebsing, Kallaya Sritunyalucksana, Olivier Decamp, Andrew P. Shinn, Ratchakorn Wongwaradechkul, Barbara Hostins
Applying Sanolife® MIC probiotic at 1-30 g/m³/day in Penaeus vannamei hatcheries significantly accelerates early larval metamorphosis, enhances postlarval growth, and reduces Vibrio loads in both water and shrimp without adverse health effects.
This 23-day study evaluated the effects of the Bacillus-based probiotic formulation Sanolife® MIC (S-MIC) at concentrations of 1, 5, and 30 g/m³/day on Pacific whiteleg shrimp (Penaeus vannamei) from nauplii to postlarval stage 15 (PL-15) under commercial hatchery conditions. Supplementation at 1 g/m³/day significantly accelerated metamorphosis to the PL-1 stage on days 9 (17.00% vs. 11.00% in controls) and 10 (74.00% vs. 69.33%). Higher concentrations (5 and 30 g/m³/day) significantly enhanced PL-6 individual larval weight compared to lower doses. Crucially, all S-MIC concentrations consistently suppressed total Vibrio counts across all developmental stages in both culture water and larvae, yielding a 2.1- to 4.6-fold reduction in water Vibrio loads by PL-15 compared to controls. Probiotic supplementation maintained high survival rates (85.7–87.0%), robust salinity stress tolerance (>98%), and normal hepatopancreas lipid vacuoles with zero morphological deformities. These results confirm concentration-specific benefits of S-MIC: 1 g/m³/day for accelerated early development and 5–30 g/m³/day for enhanced postlarval growth and biosecurity control.
Penaeus vannamei, Bacillus probiotics, Vibrio control, shrimp larviculture, hatchery management, postlarval quality, biosecurity
Commercial Bacillus probiotics can replace antibiotics in Farfantepenaeus brasiliensis postlarvae production without water exchange, achieving comparable survival, growth, postlarval quality, and Vibrio control while maintaining lower nitrite levels.
A scientific selection of Bacillus strains used as water probiotics effectively reduced pathogenic Vibrio loads and matched or exceeded prophylactic antibiotics in commercial shrimp hatcheries across Asia and Latin America.
A comprehensive review of biosecurity strategies across the shrimp value chain, covering broodstock management, pathogen exclusion, microbial management, and farm-level protocols to prevent disease outbreaks.
